| Product info: Hot Start Taq DNA polymerase |
| Name | Hot Start Taq DNA polymerase | | Cat. # | E351 | E352 | | Package, u.a. | 200 | 1000 | | Concentration, u.a./ml | 5000 | 5000 |
| Description | Hot Start Taq DNA Polymerase is complex mixture
of a thermostable 94 kD Taq DNA Polymerase purified from
E.coli recombinant strain expressing Thermus aquaticus
polymerase gene and specific monoclonal antibodies from mouse.
Hot StartTaq DNA Polymerase is inactive under conditions
of amplification reaction preparation. It can eliminate amplification
artefacts such as primer-dimer formation and mispriming
during preamplification stage and thus may provide improved
specificity when compared to standard DNA polymerases.
An advantage of Hot Start Taq DNA Polymerase is the absence
of additional heating step for polymerase activation.
Heat activation of enzyme occurs during the first denaturation step.
An inactive complex of Hot Start Taq DNA Polymerase dissociates
automatically over + 70°C, allowing activation of DNA polymerase.
Applications:
- Highly specific PCR;
- Multiplex PCR (highly recommended);
- High sensitivity applications.
| | Unit definition | One unit is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid precipitable material in 30 minutes at 74°C.
| | Reaction buffer | SE-buffer Hot Start Taq DNA polymerase, (67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.) | | Optimal temperature | 74oC | | Storage conditions | 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Nonidet P-40, 0.5 % Tween-20. Store at -20°C. | | Notes | The recommended amount of enzyme
is 1 u per 50μl of a total reaction volume.
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| Legal |
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
| | MSDS: | Download MSDS as PDF |
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