Product info: Hot Start Taq DNA polymerase


Name
Hot Start Taq DNA polymerase
Cat. #E351E352
Package, u.a.2001000
Concentration, u.a./ml50005000

DescriptionHot Start Taq DNA Polymerase is complex mixture of a thermostable 94 kD Taq DNA Polymerase purified from E.coli recombinant strain expressing Thermus aquaticus polymerase gene and specific monoclonal antibodies from mouse. Hot StartTaq DNA Polymerase is inactive under conditions of amplification reaction preparation. It can eliminate amplification artefacts such as primer-dimer formation and mispriming during preamplification stage and thus may provide improved specificity when compared to standard DNA polymerases. An advantage of Hot Start Taq DNA Polymerase is the absence of additional heating step for polymerase activation. Heat activation of enzyme occurs during the first denaturation step. An inactive complex of Hot Start Taq DNA Polymerase dissociates automatically over + 70°C, allowing activation of DNA polymerase.
Applications:
- Highly specific PCR;
- Multiplex PCR (highly recommended);
- High sensitivity applications.
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid precipitable material in 30 minutes at 74°C.
Reaction bufferSE-buffer Hot Start Taq DNA polymerase, (67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.)
Optimal temperature
74oC
Storage conditions20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Nonidet P-40, 0.5 % Tween-20. Store at -20°C.
NotesThe recommended amount of enzyme is 1 u per 50μl of a total reaction volume.
Legal Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
MSDS:Download MSDS as PDF