Recognition site | CC↑TCGAGG GGAGCT↓CC |
Source | Arthrobacter species 7M06 |
Assayed on | pUC19SE/DriI - digest |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI -digest in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | SE-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 75 - 100 | 10 - 25 | 0 - 10 | 50 - 75 | 0 - 10 | 50 |
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Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
Ligation | After 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
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Reagents Supplied with Enzyme |
10 X SE-buffer AbsI |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 65oC |
Notes | A long incubation time may result in star activity. |
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter AbsI from Arthrobacter species Recognizes Palindromic DNA Sequence 5’-CC^TCGAGG-3’ // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 2, pp 29-34, 2006
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