Product info: Abs I


Name
Abs Inew package  
Cat. #E535E536
Package, u.a.50250
Concentration, u.a./ml500-1000500-1000

Recognition site
CCTCGAGG
GGAGCTCC
SourceArthrobacter species 7M06
Assayed onpUC19SE/DriI - digest
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI -digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferSE-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10010 - 250 - 1050 - 750 - 1050
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA;0,05% Triton X-100; 50% glycerol; Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer AbsI
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesA long incubation time may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References: V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter AbsI from Arthrobacter species Recognizes Palindromic DNA Sequence 5-CC^TCGAGG-3 // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 2, pp 29-34, 2006
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322