|Assayed on|| Adenovirus -2 DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Ad2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||SE-buffer RigI (10 mM Tris-HCl (pH 8.5 at 25°C); 5 mM MgCl2; 1mM DTT.) + BSA|
|Enzyme activity (%)|
|75 - 100||50 - 75||0 - 10||10 - 25||50 - 75||10|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.|
Storage at -20°C.
Storage at -70°C is recommended for periods longer than 7 days.
|Ligation||After 3-fold overdigestion with enzyme > 95% of Ad2 DNA fragments can be ligated with T4 DNA Ligase and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 4 units of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer RigI, BSA|
|Methylation sensitivity||Blocked by mCG or GmC methylation:|
|Inactivation 20 minutes under||65oC|
|Notes||Do not use BSA for long incubation.|
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|