Product info: Rig I


Name
Rig I
Cat. #E529E530
Package, u.a.100500
Concentration, u.a./ml30003000

Recognition site
GGCCGGCC
CCGGCCGG
SourceRhizobium yangligense
Assayed on Adenovirus -2 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Ad2 DNA in 1 hour at 37C in a total reaction volume of 50 μl.
Optimal SE-bufferSE-buffer RigI (10 mM Tris-HCl (pH 8.5 at 25°C); 5 mM MgCl2; 1mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
75 - 10050 - 750 - 1010 - 2550 - 7510
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Store at -20°C.
For long term storage (more than 7 days), store at -70°C.
LigationsAfter 5-fold overdigestion with enzyme > 95% of Ad2 DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 6 units of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer RigI, BSA
Methylation sensitivityBlocked by mCG or GmC methylation:
5`-GGC(m5C)GGCC-3`/3`-CCGG(m5C)CGG-5` or
5`-GG(m5C)CGG(m5C)C-3`/3`-C(m5C)GGC(m5C)GG-5`
Inactivation 20 minutes under
65oC
NotesDo not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322