Product info: BstSN I


Name
BstSN I
Cat. #E065E066
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition site
TACGTA
ATGCAT
SourceBacillus stearothermophilus SN
Assayed onT7 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10050 - 750 - 1010 - 2550 - 7550
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at 20°C.
LigationsAfter 5-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration results in star activity
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Myakisheva, T.V., Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322