Product info: Set I


Name
Set I
Cat. #E537E538E538X
Package, u.a.20010001000
Concentration, u.a./ml5000500020000

Recognition site
ASST
TSSA
SourceE.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Assayed onDouble-stranded oligonucleotide
5`-CGAGTTTATAGCTGGGCCCAAC-3`
3`-GCTCAAATATCGACCCGGGTTG-5`
DescriptionNote! SetI cleaves a canonical site and several other sites with a weaker activity.
In the case of long incubation with SetI DNA can be digested to small oligos.
Unit definitionOne unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
25 - 5025 - 5075 - 10075 - 100100100
Optimal temperature
55oC
Storage conditions10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme, approximately 50% of the pBR322 DNA fragments can be ligated with T4 DNA Ligase and recut.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References: Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease SetI // In press