Product info: Bar I


Name
Bar I
Cat. #E547E548
Package, u.a.100500
Concentration, u.a./ml500-2000500-2000

Recognition site
(N)7GAAGNNNNNNTAC(N)12
(N)12CTTCNNNNNNATG(N)7
SourceBacillus sphaericus
Assayed onT7 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferSE-buffer 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
0 - 100 - 1025 - 5050 - 7510 - 2540
Optimal temperature
37oC
Storage conditions20 mM KH2PO4(pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer 2K
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF