Product info: M3.BstF5 I


Name
M3.BstF5 I
Cat. #M007
Package, u.a.1000
Concentration, u.a./ml10000

Recognition site
GG(m6A)TG
SourceAn E.coli strain, that carries the cloned M3.BstF5I gene from Bacillus stearothermophilus F5.
DescriptionM3.BstF5I Methyltransferase modifies the adenin residue (N6) in one strand of the recognition sequence forming 5`-GG(m6A)TG-3`/3`-CCTAC-5`.
Unit definitionOne unit of the enzyme is the amount required to protect 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 20μl against cleavage by BstF5 I restriction endonuclease.
Reaction bufferSE-buffer K + SAM, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100mM KCl; 1 mM DTT + 0,08mM SAM.
(32 mM S-adenosylmethionine supplied))
Optimal temperature
60oC
Storage conditions10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 50% glycerol. Store at -20°C.
NotesTo obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
MSDS:Download MSDS as PDF
References:Chernukhin V.A., Kuznetsov V.V., Gonchar D.A., Kashirina Yu.G., Netesova N.A., Degtyarev S. Kh. Analysis of substrate specificity and biochemical properties of DNA methyltransferase M3.BstF5I from BstF5I restriction-modification system // Biochemistry (Moscow), Vol. 75, No 1, p. 78-87 (2010). (In Russian)