Product info: T7 RNA polymerase


Name
T7 RNA polymerase
Cat. #E355E356
Package, u.a.500025000
Concentration, u.a./ml100000100000

SourceIsolated from E.coli strain that carries the cloned T7 gene I
DescriptionT7 RNA Polymerase catalyzes the synthesis of RNA in the 5 -> 3 direction in the presence of a DNA template containing a T7 phage promoter.
Applications:
-Radiolabeled RNA probe preparation
-RNA generation for in vitro translation
-RNA generation for studies of RNA structure,processing and catalysis
Unit definitionOne unit is the amount of enzyme required to incorporate 1 nmol NTP into an acid - insoluble material in 1 hour at 37C.
Reaction bufferSE-buffer RNA polymerase T7, (50 mM Tris-HCl (pH 7.5 at 25°C); 6 mM MgCl2; 10 mM Dithiothreitol; 2 mM spermidine.)
Optimal temperature
37oC
Storage conditions50 mM Tris - HCl (pH 7.5); 100 mM NaCl; 20 mM 2-mercaptoethanol; 1 mM EDTA; 0.1% Triton X-100; 50% glycerol. Store at -20C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF