Product info: AluB I


Name
AluB I
Cat. #E549E550
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition site
AGCT
TCGA
SourceArthrobacter luteus B
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
10075 - 10010 - 2510 - 2575 - 10060
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme 80% of the Lambda DNA fragments can be ligated with T4 DNA Ligase and can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Methylation sensitivityAluB I is able to cleave some methylated DNA substrates.
Inactivation 20 minutes under
65oC
NotesAluB I is an isoschizomer of Alu I.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References: V.A. Chernukhin, A. A. Boltengagen, G. V. Tarasova, V.S. Dedkov, S.Kh. Degtyarev New Restriction Endonuclease AluBI from Arthrobacter luteus B - AluI Isoshizomer, nonsensitive to Presence of 5-methylcytosine in the Recognition Sequence AGCT // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 1, pp 21-27, 2007
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322