| Product info: dNTP Mix, 0.5 mM of each, water solution, 1 ml |
| Name | dNTP Mix, 0.5 mM of each, water solution, 1 ml | | Cat. # | N024 | | Package, μmol | 2 | | Concentration, mmol/l | 2 |
| Source | Method of production: enzymatic synthesis. | | Description | Deoxynucleotide solution mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a final concentration of 0,5 mM.
| | Application | For use in molecular biology and biotechnology, including PCR, long-PCR, and for labeling and sequencing DNA. PCR-diagnostics for medicine. | | Storage conditions | Store at -20°C | | Quality control | Test for detection of endo- and exo-deoxyribonuclease and phosphatase contaminants: No detectable degradation of single-stranded and double-stranded oligonucleotide was observed after incubation of dNTP mix for 3 hour at 37°C.
Test for detection of RNase contaminants: No detectable degradation of fluorescent-marked oligonucleotide was observed after incubation of dNTP mix for 30 minunts at 37°C.
Functional test: The product has been
tested for receiving products with
length more than 15,000 base pair
nucleotides with T7 phage DNA
as a template. | | MSDS: | Download MSDS as PDF |
PCR amplification of 15.1 kb DNA fragment. Template - T7 bacteriophage DNA.
Primers:
5 ctcgcaagtctcgccgta 3`
5` cgcattgtctcgccttca 3`
Termocycler profile:
94°C - 2 min
80°C - pause, adding 1 unit TaqSE DNA Polymerase
35 cycles:
94°C - 5 sec
56°C - 10 sec
68°C - 20 min
1% TAE agarose gel
Lane 1 chemically synthesized dNTPs
Lane 2 enzyme-manufactured dNTPs
Lane 3 λ DNA BglI Digest |  |
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