|Product info: dNTP Mix, 0.5 mM of each, water solution, 1 ml|
|Name||dNTP Mix, 0.5 mM of each, water solution, 1 ml|
|Cat. #||N024 |
|Package, μmol||2 |
|Concentration, mmol/l||2 |
|Source|| Method of production: enzymatic synthesis.|
|Description||Deoxynucleotide solution mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a final concentration of 0,5 mM.
|Application||For use in molecular biology and biotechnology, including PCR, long-PCR, and for labeling and sequencing DNA. PCR-diagnostics for medicine.|
|Storage conditions||Store at -20°C|
|Quality control||Test for detection of endo- and exo-deoxyribonuclease and phosphatase contaminants: No detectable degradation of single-stranded and double-stranded oligonucleotide was observed after incubation of dNTP mix for 3 hour at 37°C.|
Test for detection of RNase contaminants: No detectable degradation of fluorescent-marked oligonucleotide was observed after incubation of dNTP mix for 30 minunts at 37°C.
Functional test: The product has been
tested for receiving products with
length more than 15,000 base pair
nucleotides with T7 phage DNA
as a template.
|MSDS:||Download MSDS as PDF|
PCR amplification of 15.1 kb DNA fragment. Template - T7 bacteriophage DNA.
5 ctcgcaagtctcgccgta 3`
5` cgcattgtctcgccttca 3`
94°C - 2 min
80°C - pause, adding 1 unit TaqSE DNA Polymerase
94°C - 5 sec
56°C - 10 sec
68°C - 20 min
1% TAE agarose gel
Lane 1 chemically synthesized dNTPs
Lane 2 enzyme-manufactured dNTPs
Lane 3 λ DNA BglI Digest