|Product info: 50 Kb DNA Ladder|
|Name||50 Kb DNA Ladder|
|Cat. #||M29||M30 |
|Package, μg||50||250 |
|Concentration, μg/ml||200||200 |
|Description||The 50 Kb DNA Ladder has a number of proprietary plasmids and phages DNA which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose pulsed-field
gel electrophoresis (PFGE).|
|Length of the DNA fragments (Base Pairs) |
| Base Pairs ||DNA Mass, ng|| Base Pairs ||DNA Mass, ng
|Storage conditions||10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl. Store at -20°C.|
|Notes||The 50 Kb DNA Ladder was not designed for precise quantification of DNA mass, but can be used for approximating the mass of DNA in comparably intense samples of similar size.|
We recommend loading 0,5-1 μg of the DNA Ladder per line.
We recommend to add a loading dye in amount 1/10 from the DNA Ladder volume.
This DNA Ladder can be visualized by ethidium bromide staining as well as GelRed(TM) Nucleic Acid gel staining.
|MSDS:||Download MSDS as PDF|
|0,5 μg of 50 Kb DNA Ladder visualized by ethidium bromide staining on 0,8% TAE agarose gel after PFGE separation.|