Product info: 50 Kb DNA Ladder


Name
50 Kb DNA Ladder
Cat. #M29M30
Package, μg50250
Concentration, μg/ml200200

DescriptionThe 50 Kb DNA Ladder has a number of proprietary plasmids and phages DNA which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose pulsed-field gel electrophoresis (PFGE).
Length of the DNA fragments (Base Pairs)
Base Pairs DNA Mass, ng Base Pairs DNA Mass, ng
48502703000140
3993670250050
2473070200045
1520670150035
10000451000150
80004575045
60004550020
50004525010
400045   

Storage conditions10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl. Store at -20C.
NotesThe 50 Kb DNA Ladder was not designed for precise quantification of DNA mass, but can be used for approximating the mass of DNA in comparably intense samples of similar size.
We recommend loading 0,5-1 μg of the DNA Ladder per line.
We recommend to add a loading dye in amount 1/10 from the DNA Ladder volume.
This DNA Ladder can be visualized by ethidium bromide staining as well as GelRed(TM) Nucleic Acid gel staining.
MSDS:Download MSDS as PDF
0,5 μg of 50 Kb DNA Ladder visualized by ethidium bromide staining on 0,8% TAE agarose gel after PFGE separation.