Product info: CciN I


Name
CciN IBlocked by 
G(5mC)GGC(5mC)GC methylation  
Cat. #E203E204E203TE204T
Package, u.a.20010002001000
Concentration, u.a./ml2000-50002000-50002000-50002000-5000

Recognition site
GCGGCCGC
CGCCGGCG
SourceCurtobacterium citreus N
Assayed onAdenovirus-2 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
25 - 5050 - 7575 - 10075 - 100100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y.
For E203T and E204T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322