Product info: CciN I

CciN IBlocked by 
G(5mC)GGC(5mC)GC methylation  
Cat. #E203E204
Package, u.a.5002500
Concentration, u.a./ml5000-100005000-10000

Recognition site
SourceAn E.coli strain, that carries the cloned gene CciNI from Curtobacterium citreus N
Assayed onpUC-AD plasmid DNA.
pUC-AD (22562 bp) was obtained by a ligation of the vector pUC19/XbaI and a XbaI-fragment of Adenovirus-2 DNA, 19876 bp in length. pUC-AD contains 6 recognition sites of CciNI.
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pUC-AD DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
25 - 5050 - 7575 - 10075 - 100100100
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA pUC-AD with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y.
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
NotesHigh enzyme concentration may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995).