|Source||An E.coli strain, that carries the cloned gene CciNI from Curtobacterium citreus N|
|Assayed on||pUC-AD plasmid DNA. |
pUC-AD (22562 bp) was obtained by a ligation of the vector pUC19/XbaI and a XbaI-fragment of Adenovirus-2 DNA, 19876 bp in length. pUC-AD contains 6 recognition sites of CciNI.
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC-AD DNA in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
|Enzyme activity (%)|
|25 - 50||50 - 75||75 - 100||75 - 100||100||100|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.|
|Ligation||After 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of DNA pUC-AD with 10 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer Y.|
|Methylation sensitivity||Blocked by CG methylation.|
|Inactivation 20 minutes under||65oC|
|Notes||High enzyme concentration may result in star activity.
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995).