| Name | | EcoR I |  |
| | Cat. # | E057 | E058 | E057X | E058X | E057T | E058T | | Package, u.a. | 5000 | 25000 | 5000 | 25000 | 5000 | 25000 | | Concentration, u.a./ml | 20000 | 20000 | 50000 | 50000 | 20000 | 20000 |
| Recognition site | G↑AATTC
CTTAA↓G | | Source | E.coli strain that carries the cloned EcoR I gene from Escherichia coli | | Assayed on | Lambda DNA | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | | Optimal SE-buffer | SE-buffer EcoRI (100 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA | | Enzyme activity (%) | | B | G | O | W | Y | Rose | | 50 - 75 | 75 - 100 | 75 - 100 | 75 - 100 | 50 - 75 | 50 |
| | Optimal temperature | 37oC | | Storage conditions | 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerolerol; Store at -20°C. | | Ligations | After 40-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer EcoRI, BSA (except E057m, E057T and E058T).
For E057T and E058T an additional 10X SE-buffer ROSE is supplied.
| | Methylation sensitivity | not tested | | Inactivation 20 minutes under | 65oC | | Notes | High enzyme concentration and using of nonoptimal buffer may result in star activity.
Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
| | Quality control | Restriction Endonucleases: Quality Control | | MSDS: | Download MSDS as PDF | | References: | Albertsen, H.M., Le Paslier, D., Abderrahim, H., Dausset, J., Cann, H., Cohen, D. Nucleiv Acids Res. 17: 808 (1989).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
| Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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