Product info: EcoR V

EcoR VNew package  
Cat. #E059E060E059TE060TE059m
Package, u.a.200010000200010000500
Concentration, u.a./ml2000020000200002000020000

Recognition site
SourceE.coli strain, that carries the cloned gene EcoRV from Escherichia coli
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
0 - 1025 - 5050 - 7510025 - 5050
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme fro 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W, BSA (except E059m, E059T and E060T).
For E059T and E060T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivitynot tested
Inactivation 20 minutes under
NotesHigh enzyme concentration results in star activity.
Do not use BSA for long incubation
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Kholmina, .V., Rebentish, B.A., Skoblov, Yu.S., Dokl.Akad. Nauk SSSR 253: 495-497 (1980).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322