Product info: Fau I

Fau IBlocked by 
CC(5mC)GC methylation  
Cat. #E209E210
Package, u.a.100500
Concentration, u.a./ml20002000

Recognition site
SourceE.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
10025 - 500 - 100 - 1050 - 7550
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 55°C
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
NotesThe minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322