| Recognition site | GACGT↑C
C↓TGCAG |
| Source | E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Reaction buffer | SE-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer Y |
| Inactivation 20 minutes under | 65oC |
| Notes | High enzyme concentration may result in star activity. |
| MSDS: | Download MSDS as PDF |
| References: | Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Nucleic Acid Res. 10:5747-5752(1982)
|