Product info: Aat II

Aat II
Cat. #E287m
Package, u.a.250
Concentration, u.a./ml10000

Recognition site
SourceE.coli strain, that carries the cloned Aat II gene from Acetobacter aceti
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction bufferSE-buffer Y
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Inactivation 20 minutes under
NotesHigh enzyme concentration may result in star activity.
MSDS:Download MSDS as PDF
References:Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Nucleic Acid Res. 10:5747-5752(1982)