Recognition site | GACGT↑C C↓TGCAG |
Source | E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti |
Assayed on | Lambda DNA |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Reaction buffer | SE-buffer Y |
Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C. |
Ligation | After 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
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Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Inactivation 20 minutes under | 65oC |
Notes | High enzyme concentration may result in star activity. |
MSDS: | Download MSDS as PDF |
References: | Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Nucleic Acid Res. 10:5747-5752(1982)
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