|Source||Acinetobacter calcoaceticus 65|
|Assayed on||Lambda DNA(dcm-)|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Reaction buffer||SE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.|
|Ligations||After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer W|
For E003T and E004T an additional 10X SE-buffer ROSE is supplied.
|Inactivation 20 minutes under||65oC|
|MSDS:||Download MSDS as PDF|
|References:||Prichodko, G.G., Rechkunova, N.I., Repin, V.E., Degtyarev, S.Kh., Sib. Biol. J. 1: 59-60 (1991).|