Name | Ahl I | Cat. # | E173m | Package, u.a. | 250 | Concentration, u.a./ml | 10000 |
Recognition site | A↑CTAGT TGATC↓A | Source | Alteromonas haloplanktis SP | Assayed on | T7 DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | Reaction buffer | SE-buffer B + BSA | Optimal temperature | 37oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | Ligation | After 20-fold overdigestion with enzyme more than 90% of T7 DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a. of enzyme for 16 hours at 37°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer B, BSA
| Inactivation 20 minutes | No | Notes | To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation. | MSDS: | Download MSDS as PDF | References: | Myakisheva, T.V., Belichenko, O.A., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (2000).
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