Product info: Bgl II


Name
Bgl IINot blocked by 
 AG(6mA)TCT methylation    
Cat. #E027m
Package, u.a.500
Concentration, u.a./ml10000

Recognition site
AGATCT
TCTAGA
SourceIsolated from an E.coli strain that carries the cloned Bgl II gene from Bacillus globigii
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction bufferSE-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O.
For E027T and E028T an additional 10X SE-buffer ROSE is supplied.
Inactivation 20 minutes under
80oC
MSDS:Download MSDS as PDF
References:Pirrotta, V. Nucleic Acids Res. 3: 1747-1760 (1976).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007