| Name | | Bme18 I |  |
| | Cat. # | E029m | | Package, u.a. | 500 | | Concentration, u.a./ml | 10000 |
| Recognition site | G↑GWCC
CCWG↓G | | Source | Bacillus megaterium 18 | | Assayed on | Lambda DNA | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | | Reaction buffer | SE-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) | | Optimal temperature | 37oC | | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C. | | Ligation | After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer O | | Inactivation 20 minutes under | 65oC | | MSDS: | Download MSDS as PDF | | References: | Degtyarev S.K., Rechkunova N.I., Grinev A.A., Dedkov V.S. Izv. Sib. Otd. Akad. Nauk SSSR 15: 25-26 (1989).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
|
| |