| Name | | BstDE I |   |
| | Cat. # | E227m | | Package, u.a. | 500 | | Concentration, u.a./ml | 10000 |
| Recognition site | C↑TNAG
GANT↓C | | Source | Bacillus stearothermophilus DE | | Assayed on | Lambda DNA | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. | | Reaction buffer | SE-buffer G | | Optimal temperature | 60oC | | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. | | Ligation | After 30-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 60 u.a. of enzyme for 16 hours at 60°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer G | | Inactivation 20 minutes under | 80oC | | Notes | At 37°C activity is 10% from maximum | | MSDS: | Download MSDS as PDF | | References: | Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).
 Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
|
| |