Name | EcoR V | |
| Cat. # | E059m | Package, u.a. | 500 | Concentration, u.a./ml | 20000 |
Recognition site | GAT↑ATC CTA↓TAG | Source | E.coli strain, that carries the cloned gene EcoRV from Escherichia coli | Assayed on | Lambda DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | Reaction buffer | SE-buffer W + BSA | Optimal temperature | 37oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | Ligation | After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme fro 16 hours at 37°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer W, BSA
| Inactivation 20 minutes under | 80oC | Notes | High enzyme concentration results in star activity.
Do not use BSA for long incubation
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
| MSDS: | Download MSDS as PDF | References: | Kholmina, .V., Rebentish, B.A., Skoblov, Yu.S., et.al. Dokl.Akad. Nauk SSSR 253: 495-497 (1980).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
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