Product info: EcoR V Name EcoR V    Cat. # E059m Package, u.a. 500 Concentration, u.a./ml 20000 Recognition site GAT↑ATC CTA↓TAG Source E.coli strain, that carries the cloned gene EcoRV from Escherichia coli Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Reaction buffer SE-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA) Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme fro 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA Inactivation 20 minutes under 80oC Notes High enzyme concentration results in star activity. Do not use BSA for long incubation To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. MSDS: Download MSDS as PDF References: Kholmina, .V., Rebentish, B.A., Skoblov, Yu.S., et.al. Dokl.Akad. Nauk SSSR 253: 495-497 (1980).  Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007