Product info: EcoR V


Name
EcoR VNew package  
Cat. #E059m
Package, u.a.500
Concentration, u.a./ml20000

Recognition site
GATATC
CTATAG
SourceE.coli strain, that carries the cloned gene EcoRV from Escherichia coli
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction bufferSE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme fro 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W, BSA (except E059m, E059T and E060T).
For E059T and E060T an additional 10X SE-buffer ROSE is supplied.
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration results in star activity.
Do not use BSA for long incubation
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
MSDS:Download MSDS as PDF
References:Kholmina, .V., Rebentish, B.A., Skoblov, Yu.S., et.al. Dokl.Akad. Nauk SSSR 253: 495-497 (1980).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007