| Name | | Hind III |  |
| | Cat. # | E073m | | Package, u.a. | 500 | | Concentration, u.a./ml | 10000 |
| Recognition site | A↑AGCTT
TTCGA↓A | | Source | E.coli strain, that carries the cloned gene Hind III from Haemophilus influenzae Rd | | Assayed on | Lambda DNA | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | | Reaction buffer | SE-buffer W + BSA | | Optimal temperature | 37oC | | Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | | Ligation | After 50-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer W, BSA
| | Inactivation 20 minutes under | 80oC | | Notes | To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
| | MSDS: | Download MSDS as PDF | | References: | Old, R., Murray, K., Roizes, G. J. Mol. Biol. 92: 331-339 (1975).
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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