Name | Hinf I | |
| Cat. # | E075m | Package, u.a. | 500 | Concentration, u.a./ml | 10000 |
Recognition site | G↑ANTC CTNA↓G | Source | E.coli strain, that carries the cloned gene HinfI from Haemophilus influenzae | Assayed on | Lambda DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | Reaction buffer | SE-buffer O | Optimal temperature | 37oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | Ligation | After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer O.
For E075T and E076T an additional 10X SE-buffer ROSE is supplied.
| Inactivation 20 minutes under | 80oC | MSDS: | Download MSDS as PDF | References: | Hutchison, C.A., Barrell, B.G. Unpublished observations. (1992)
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
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