Product info: Hpa I Name Hpa I Cat. # E077m Package, u.a. 250 Concentration, u.a./ml 5000 Recognition site GTT↑AAC CAA↓TTG Source E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Reaction buffer SE-buffer Y Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Ligation After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y. For E077T and E078T an additional 10X SE-buffer ROSE is supplied. Inactivation 20 minutes under 65oC Notes High enzyme concentration results in star activity. MSDS: Download MSDS as PDF References: Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973). Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).