| Recognition site | GTT↑AAC
CAA↓TTG |
| Source | E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Reaction buffer | SE-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligation | After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer Y.
For E077T and E078T an additional 10X SE-buffer ROSE is supplied.
|
| Inactivation 20 minutes under | 65oC |
| Notes | High enzyme concentration results in star activity. |
| MSDS: | Download MSDS as PDF |
| References: | Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973).
Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).
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