Product info: Mfe I


Name
Mfe I
Cat. #E295m
Package, u.a.500
Concentration, u.a./ml10000

Recognition site
CAATTG
GTTAAC
SourceE.coli strain, that carries the cloned gene Mfe I from Mycoplasma fermentans.
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Reaction bufferSE-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Inactivation 20 minutes
No
NotesTo obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
MSDS:Download MSDS as PDF