Name | | Cat. # | E275m | Package, u.a. | 150 | Concentration, u.a./ml | 5000 |
Recognition site | A↑CATGT TGTAC↓A | Source | E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45 | Assayed on | T7 DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | Reaction buffer | SE-buffer O | Optimal temperature | 37oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. | Ligation | After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 37°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer O | Inactivation 20 minutes under | 65oC | Notes | The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13. | MSDS: | Download MSDS as PDF | References: | Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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