Product info: Pci I Name Pci I          Cat. # E275m Package, u.a. 150 Concentration, u.a./ml 5000 Recognition site A↑CATGT TGTAC↓A Source E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45 Assayed on T7 DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Reaction buffer SE-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O Inactivation 20 minutes under 65oC Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13. MSDS: Download MSDS as PDF References: Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998).  V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006