|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Reaction buffer||SE-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.|
|Ligations||After 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer B|
|Inactivation 20 minutes under||65oC|
|Notes||The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.|
|MSDS:||Download MSDS as PDF|
|References:||Abdurashitov, M.A., Belichenko, O.A., Lebedeva, N.A., Degtyarev, S.Kh.
Biokhimiia 64: 574-576 (1999).|