|Source||Pseudomonas species 124B|
|Assayed on||Lambda DNA (HindIII-digest)|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Reaction buffer||SE-buffer G, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Ligation||After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer G.|
For E107T and E108T an additional 10X SE-buffer ROSE is supplied.
|Inactivation 20 minutes under||80oC|
|MSDS:||Download MSDS as PDF|
|References:||Degtyarev, S.Kh., Dedkov, V.S., Rechkunova, N.I., Morgan, R. Unpublished observations (1994).|