| Name | | Rsa I |  |
| | Cat. # | E113m | | Package, u.a. | 500 | | Concentration, u.a./ml | 10000 |
| Recognition site | GT↑AC
CA↓TG | | Source | Rhodopseudomonas sphaeroides | | Assayed on | Lambda DNA | | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | | Reaction buffer | SE-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) | | Optimal temperature | 37oC | | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C. | | Ligation | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
| | Reagents Supplied with Enzyme |
10 X SE-buffer B | | Inactivation 20 minutes | No | | MSDS: | Download MSDS as PDF | | References: | Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F. J.Bacteriol. 142: 380-383 (1980).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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