Product info: Sfr274 I Name Sfr274 I      Cat. # E125m Package, u.a. 500 Concentration, u.a./ml 10000 Recognition site C↑TCGAG GAGCT↓C Source Streptomyces fradiae 274 Assayed on Lambda DNA (Hind III-digest) Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 50°C in a total reaction volume of 50 μl. Reaction buffer SE-buffer B Optimal temperature 50oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.. Store at -20°C. Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer B. For E125T and E126T an additional 10X SE-buffer ROSE is supplied. Inactivation 20 minutes under 65oC Notes At 37°C activity is about 70% from maximum. MSDS: Download MSDS as PDF References: Puchkova, L.I., Krivopalova, G.N., Andreeva, I.S., Selina, A.V., Serov, G.D., Rechkunova, N.I., Degtyarev, S.Kh. Izv. Sib. Otd. Akad. Nauk SSSR 1: 32-34 (1990).