|Source||E.coli strain that carries the cloned Sma I gene from Serratia marcescens|
|Assayed on||Lambda DNA (HindIII-digest)|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl.|
|Reaction buffer||SE-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Ligation||After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 25°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer Y.|
For E177T and E178T an additional 10X SE-buffer ROSE is supplied.
|Inactivation 20 minutes under||65oC|
|MSDS:||Download MSDS as PDF|
|References:||Endow, S.A., Roberts, R.I., J. Mol. Biol. 112: 521-529 (1977).|