|Cat. #||E199m |
|Package, u.a.||250 |
|Concentration, u.a./ml||10000 |
|Source||E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9|
|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.|
|Reaction buffer||SE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)|
|Storage conditions||10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20°C.|
|Ligations||After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer W|
|Inactivation 20 minutes under||80oC|
|MSDS:||Download MSDS as PDF|
|References:||Prichodko, E.A., Rechkunova, N.I., Degtyarev, S.Kh. Sib. Biol. J. 1:57-59 (1991).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006