| Recognition site | T↑CTAGA
AGATC↓T |
| Source | E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii |
| Assayed on | Lambda DNA (dam-/HindIII-digest) |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Reaction buffer | SE-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C. |
| Ligation | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer O, BSA (except E141m, E141T and E142T).
For E141T and E142T an additional 10X SE-buffer ROSE is supplied.
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| Inactivation 20 minutes under | 65oC |
| Notes | To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation. |
| MSDS: | Download MSDS as PDF |
| References: | Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
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