Product info: Sal I


Name
Sal I
Cat. #E115TE116T
Number of reactions2001000
Volume, μl2001000

Recognition site
GTCGAC
CAGCTG
SourceAn E.coli strain that carries the cloned Sal I gene from Streptomyces albus
Assayed onLambda DNA (HindIII-digest), plasmid DNA
DescriptionTurbo Sal I is used for a short time (15 min) of 1 μg DNA digestion in SE-Buffer O in 50 μl of reaction mixture.
Applications:
- Fast DNA analysis
- Fast preparation of vectors for cloning
- Double digestion
Reaction bufferSE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter digestion with 1 μl of Turbo Sal I, approximately 95% of the DNA fragments can be ligated with high-activity T4 DNA Ligase and recut.
Non-specific hydrolisisNo detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 1 μl of restriction endonuclease for 3 hours.
Reagents Supplied with Enzyme 10 X SE-buffer O
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesEnzyme Properties:
1 μl of Turbo Sal I cuts 1 μg of DNA in 1x SE-Buffer O in 15 min (assayed on Lambda/HindIII and plasmid DNA). A short time of DNA digestion requires high quality purification of DNA sample (PCR fragments should be purified after amplification).
Please note that supercoiled plasmid DNA and PCR fragments may have varying rates of cleavage and sometimes need more time to be completely digested.
Standard protocol of Turbo reaction:
20 μl of the reaction volume:
10x SE-Buffer O - 2 μl
DNA - 0.2-1 μg
Nuclease-free water - to 20 μl
+ 1 μl of Turbo Sal I
Mix by pipette tip carefully.
Incubate at 37°C for 15 min.
MSDS:Download MSDS as PDF
References:Arrand, J.R., Myers, P.A., Roberts, R.J., J. Mol. Biol. 118: 113-122 (1978).