Product info: Tru9 I


Name
Tru9 INew package  Blocked by TTA(6mA) methylation    
Cat. #E199TE200T
Number of reactions25125
Volume, μl25125

Recognition site
TTAA
AATT
SourceAn E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9
Assayed onLambda DNA, plasmid DNA
DescriptionTurbo Tru9 I is used for short time (10 min) DNA digestion in universal (ROSE) SE-Buffer.
Reaction Original SibEnzyme (ROSE) Buffer is a specially designed universal reaction buffer for the most Restriction Endonucleases. ROSE Buffer is perfect for DNA cleavage with SE Turbo Restriction Endonucleases and for double digestion.
Applications:
- Fast DNA analysis
- Fast preparation of vectors for cloning
- Double digestion
Reaction bufferSE-buffer ROSE
Optimal temperature
65oC
Storage conditions10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20°C.
LigationAfter digestion with 1 μl of Turbo Tru9 I, approximately 95% of the DNA fragments can be ligated with high-activity T4 DNA Ligase and recut.
Non-specific hydrolisisNo detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 1 μl of restriction endonuclease for 3 hours.
Reagents Supplied with Enzyme 10 X SE-buffer ROSE
Methylation sensitivityBlocked by TTAmA methylation .
Inactivation 20 minutes under
80oC
NotesEnzyme Properties:
1 μl of Turbo Tru9 I cuts 1 μg of DNA in 1x SE-Buffer ROSE in 10 min (assayed on Lambda and plasmid DNA). A short time of DNA digestion requires high quality purification of DNA sample (PCR fragments should be purified after amplification).
Please note that supercoiled plasmid DNA and PCR fragments may have varying rates of cleavage and sometimes need more time to be completely digested.
Standard protocol of Turbo reaction:
20 μl of the reaction volume:
10x SE-Buffer ROSE - 2 μl
DNA - 0.2-1 μg
Nuclease-free water - to 20 μl
+ 1 μl of Turbo Tru9 I
Mix by pipette tip carefully.
Incubate at 65°C for 10 min.
References:Prichodko, E.A., Rechkunova, N.I., Degtyarev, S.Kh. Sib. Biol. J. 1:57-59 (1991).
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006