|Product info: pHspAI10/DriI+M.Fsp4HI |
|Cat. #||D21 |
|Package, μg||10 |
|Concentration, μg/ml||200 |
|Source||pHspAI10 is isolated from E.coli (dam+,dcm+) by a standard plasmid purification procedure.|
|Description||DNA pHspAI10/DriI+M.Fsp4HI is a plasmid pHspAI10, which is linearized with DriI, and, additionally, modified with Fsp4HI DNA methyltransferase. pHspAI10 carries a gene of HspAI DNA methyltransferase, that modifies the sequence
5`-GCGC-3`, producing 5`-G(5mC)GC-3`.|
M.Fsp4HI modifies the sequence 5`-GCNGC-3`, producing 5`-G(5mC)NGC-3`.
A substrate pHspAI10/DriI+M.Fsp4HI includes one site
5`-G(5mC)G(5mC)NG(5mC)G(5mC)-3` /3`- (5mC)G(5mC)GN(5mC)G(5mC)G-5`.
|Storage conditions||10 mM Tris-HCl (pH 8.0); 1 mM EDTA. Store at - 20°C.|
|MSDS:||Download MSDS as PDF|