Product info: GlaI-PCR Assay Kit

GlaI-PCR Assay Kit
Cat. #K011SK011L
Package, reactions2001000

Description Instruction manual: Download as PDF.

The following reagents are supplied with this product:
  1. 1X  TE Buffer
  2. 10X SE TMN Buffer
  3. BSA, 10 mg/mL
  4. MD DNA-endonuclease ( 20 u/µL)
  5. β -mercaptoethanol (200ěĚ)
  6. 10X SE GLAD Buffer
  7. dNTP Mix, 10 mM each
  8. SP Taq DNA Polymerase, 5 u/µL
  9. Control DNA L-68, 18 ng/µL
  10. Control DNA HeLa, 18 ng/µL
  11. Control mouse DNA, 18 ng/ µL
  12. Control RARB mix (primers + TaqMan probe), 10 µM each
  13. DNA phage λ, 18 ng/µL
  14. DNA Raji, 18 ng/µL
ApplicationThe kit is developed for determination of methylation status of human and mammalian DNA fragment with a real-time PCR and for identification of unmethylated RCGY sites within it.
Storage conditionsStore at -20°C.
NotesReaction requires:
1. DNA sample,
2. Genome primer and TaqMan probe designed for DNA region of interest,
References:1. Handa, V., and Jeltsch A. “Profound sequence preference of Dnmt3a and Dnmt3b mammalian DNA methyltransferases shape the human epigenome” // 2005, J. Mol. Biol. 348, 1103-1112
2. Tarasova G. V., Nayakshina T. N., Degtyarev S. Kh. Substrate specificity of new methyl-directed DNA endonuclease GlaI. // BMC Molecular Biology 2008, 9:7
3. EV Dubinin, AG Akishev, MA Abdurashitov, SB Oleynikova, VL Sitko, and S Kh Degtyarev. Real time GlaI-PCR assay of regulation regions of human genes HDAC4, RARB and URB1// Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol 7(2), p.p. 667-676 (2016).
4. Alexander G. Akishev, Danila A. Gonchar, Murat A. Abdurashitov and Sergey Kh. Degtyarev Epigenetic typing of human cancer cell lines by BlsI- and GlaI-PCR assays.// Ovchinnikov bulletin of biotechnology and physical and chemical biology V.7, No 3, pp 5-16, 2011
5. Gonchar D.A., Akishev A.G., Degtyarev S.Kh. BlsI- and GlaI-PCR assays – a new method of DNA methylation study // Ovchinnikov bulletin of biotechnology and physical and chemical biology , V.6, No 1, p. 5-12 (2010)