Product info: KroN I


Name
KroN I
Cat. #E599E600
Package, u.a.100500
Concentration, u.a./ml20002000

Recognition site
GCCGGC
CGGCCG
SourceKocurea rosea 56
Assayed onpSXH7 plasmid DNA (13092 bp):
pSXH7 was obtained by a ligation of the vector pUC19/BamHI and a BstX2I-fragment of bacterial DNA Sporosarcina species 9 (G+C contents of 70-76 mol%). The plasmid contains 42 recognition sites of KroNI.
Unit definitionOne unit is defined as the amount of enzyme required to digest 1 μg of pSXH7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10075 - 10010 - 2525 - 5075 - 10050
Optimal temperature
37oC
Storage conditions20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDT A, 7 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol
LigationAfter 3-fold overdigestion with KroN I, 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis-
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivityBlocked by CG methylation 5'-GC(5mC)GGC-3'/3'-CGG(5mC)CG-5'.
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF