Product info: AccB7 I Name AccB7 I    Cat. # E179 E180 Package, u.a. 200 1000 Concentration, u.a./ml 5000 5000 Recognition site CCANNNN↑NTGG GGTN↓NNNNACC Source Acinetobacter calcoaceticus B7 Assayed on Lambda DNA (dcm-) Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 10 - 25 100 25 - 50 50 - 75 50 - 75 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity Blocked by overlapping Dcm methylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG. Inactivation 20 minutes under 65oC Notes High enzyme concentration may result in star activity. Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).