Recognition site | CCANNNN↑NTGG GGTN↓NNNNACC |
Source | Acinetobacter calcoaceticus B7 |
Assayed on | Lambda DNA (dcm-) |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 10 - 25 | 100 | 25 - 50 | 50 - 75 | 50 - 75 | 100 |
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Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
Ligations | After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
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Reagents Supplied with Enzyme |
10 X SE-buffer G |
Methylation sensitivity | Blocked by overlapping Dcm methylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG. |
Inactivation 20 minutes under | 65oC |
Notes | High enzyme concentration may result in star activity. |
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).
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