Product info: Fok I


Name
Fok I
Cat. #E247E248
Package, u.a.100500
Concentration, u.a./ml1000-20001000-2000

Recognition site
GGATG(N)9
CCTAC(N)13
SourceE.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 7525 - 5025 - 50100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesOverdigestions of > 5 units of Fok I per 1μg of DNA and incubation times > 2 hours are not recommended.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Sugisaki, H., Kanazawa, S., Gene 16: 73-78 (1981).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322