Recognition site | GGATG(N)9↑ CCTAC(N)13↓ |
Source | An E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites |
Assayed on | Lambda DNA |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 50 - 75 | 50 - 75 | 25 - 50 | 25 - 50 | 100 | 100 |
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Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
Ligation | After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C
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Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 65oC |
Notes | Overdigestions of > 5 units of Fok I per 1μg of DNA and incubations > 2 hours are not recommended. |
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Sugisaki, H., Kanazawa, S., Gene 16: 73-78 (1981).
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