Product info: Hae III

Hae IIINew package    
Cat. #E067E068E068X
Package, u.a.20001000010000
Concentration, u.a./ml100001000050000

Recognition site
SourceAn E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
75 - 10010025 - 5050 - 7550 - 75100
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivityBlocked by methylation: 5`-GG(5mC)C-3`/ 3`-C(5mC)GG-5`.
Inactivation 20 minutes under
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Middleton, J.H., Edgell, M.H., Hutchison, C.A. J. Virol. 10:42-50 (1972).
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
 M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 29-38, 2006