Product info: AccBS I


Name
AccBS I
Cat. #E007E008
Package, u.a.10005000
Concentration, u.a./ml5000-200005000-20000

Recognition site
GAGCGG
CTCGCC
SourceAcinetobacter calcoaceticus BS
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10075 - 10025 - 5025 - 50100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Kileva, E.V., Myakisheva, T.V., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.Kh., Prikl. Biochim. Microbiol., 5(33): 556-558 (1997).
 M. A. Abdurashitov, E. V. Kileva, T. V. Myakisheva, V. S. Dedkov, A. V. Shevchenko, and S. Kh. Degtyarev AccBSI: A New Restriction Endonuclease from Acinetobacter calcoaceticus BS // APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol. 33, No. 5, pp. 496-498, 1997
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322