Product info: AccBS I Name AccBS I Cat. # E007 E008 Package, u.a. 1000 5000 Concentration, u.a./ml 10000 10000 Recognition site GAG↑CGG CTC↓GCC Source Acinetobacter calcoaceticus BS Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 75 - 100 75 - 100 25 - 50 25 - 50 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y Methylation sensitivity not tested Inactivation 20 minutes under 65oC Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Abdurashitov, M.A., Kileva, E.V., Myakisheva, T.V., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.Kh., Prikl. Biochim. Microbiol., 5(33): 556-558 (1997).  M. A. Abdurashitov, E. V. Kileva, T. V. Myakisheva, V. S. Dedkov, A. V. Shevchenko, and S. Kh. Degtyarev AccBSI: A New Restriction Endonuclease from Acinetobacter calcoaceticus BS // APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol. 33, No. 5, pp. 496-498, 1997 Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322