Name | AccBS I | Cat. # | E007 | E008 | Package, u.a. | 1000 | 5000 | Concentration, u.a./ml | 10000 | 10000 |
Recognition site | GAG↑CGG CTC↓GCC | Source | Acinetobacter calcoaceticus BS | Assayed on | Lambda DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) | Enzyme activity (%) | B | G | O | W | Y | Rose | 75 - 100 | 75 - 100 | 25 - 50 | 25 - 50 | 100 | 100 |
| Optimal temperature | 37oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. | Ligation | After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer Y | Methylation sensitivity | not tested | Inactivation 20 minutes under | 65oC | Quality control | Restriction Endonucleases: Quality Control | MSDS: | Download MSDS as PDF | References: | Abdurashitov, M.A., Kileva, E.V., Myakisheva, T.V., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.Kh., Prikl. Biochim. Microbiol., 5(33): 556-558 (1997).
M. A. Abdurashitov, E. V. Kileva, T. V. Myakisheva, V. S. Dedkov, A. V. Shevchenko, and S. Kh. Degtyarev AccBSI: A New Restriction Endonuclease from Acinetobacter calcoaceticus BS // APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol. 33, No. 5, pp. 496-498, 1997
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