Product info: Mox20 I


Name
Mox20 I  
Cat. #E301E302
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition site
TGGCCA
ACCGGT
SourceMicrobacterium oxydans
Assayed onLanbda DNA (dcm-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5010075 - 10025 - 5075
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. More complete ligation can be reached by using 10% PEG
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O
Methylation sensitivityCleaved of DNA is impaired by overlapping Dcm methylation(CmCWGG): TGGCCAGG.
Inactivation 20 minutes
No
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Chernov, A.P., Belichenko, O.A., Rechkunova, N.I., Andreeva, I.S., Repin, V.E., Degtyarev, S.K. Russian Patent Office (1993). SU 1806191 A3
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322