Recognition site | CMG↑CKG GKC↓GMC |
Source | Moraxella species A1 |
Assayed on | Lambda DNA |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA |
Enzyme activity (%) | B | G | O | W | Y | Rose | 10 - 25 | 75 - 100 | 10 - 25 | 25 - 50 | 100 | 100 |
|
Optimal temperature | 37oC |
Storage conditions | 20 mM Tris-HCl (pH 7.6); 300 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 10 mM MgCl2, 200 μg/ml BSA, 50% glycerol. Store at -20°C. Storage at -70°C is recommended for periods longer than 30 days. |
Ligation | After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
|
Reagents Supplied with Enzyme |
10 X SE-buffer Y, BSA |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 65oC |
Notes | To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation. |
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Degtyarev, S.K. Unpublished observations.
|