Product info: MspR9 I


Name
MspR9 Inew package  Blocked by 
C(5mC)NGG methylation  
Cat. #E175E176
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition site
CCNGG
GGNCC
SourceMicrococcus species R9
Assayed onLambda DNA (dcm-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 7510050 - 7575 - 100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 μg/ml BSA; and 50% glycerol; Store at -20°C.
LigationsAfter 2-fold MspR9 I overdigestion none of the DNA fragments can be ligated.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O
Methylation sensitivityBlocked by overlapping Dcm methylation(CmCWGG): CCAGG and CCTGG.
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Repin, V.E., Andreeva, I.S., Kileva, E.V., Shevchenko, A.V., Abdurashitov, M.A., Repin, M.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 33: 284-286 (1997).