Product info: Nru I


Name
Nru IBlocked by 
TCGCG(6mA) methylation  
Cat. #E099E100
Package, u.a.5002500
Concentration, u.a./ml5000-100005000-10000

Recognition site
TCGCGA
AGCGCT
SourceNocardia rubra
Assayed onLambda DNA (dam-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
0 - 1010 - 2575 - 10010010 - 25100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme about 20% of the DNA fragments can be ligated. In the presence of 10% PEG ligation is better.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC): TCGCGATC.
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Comb, D.G., Schildkraut, I., Greenough, L. Unpublished observations.
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322