SE-buffers activity and inactivation temperature (20 minutes)


Enzyme Recognition sequence Optimal buffer BSA % Activity Optimal temperature, °Ρ Inactivation, °Ρ
B G O W Y Rose
Aat II
GACGTC
CTGCAG
Y-10 - 2525 - 5010 - 2525 - 50100503765
Abs I
CCTCGAGG
GGAGCTCC
*-75 - 10010 - 250 - 1050 - 750 - 10503765
Acc16 I
TGCGCA
ACGCGT
W-50 - 7575 - 10025 - 5010075 - 100703765
Acc36 I
ACCTGC(N)4
TGGACG(N)8
Y-25 - 5025 - 5050 - 7550 - 751001003765
Acc65 I
GGTACC
CCATGG
W-10 - 2525 - 5075 - 10010010 - 251003765
AccB1 I
GGYRCC
CCRYGG
K+50 - 7510 - 2510 - 2575 - 10050 - 75303765
AccB7 I
CCANNNNNTGG
GGTNNNNNACC
G-10 - 2510025 - 5050 - 7550 - 751003765
AccBS I
GAGCGG
CTCGCC
Y-75 - 10075 - 10025 - 5025 - 501001003765
Acl I
AACGTT
TTGCAA
Y+0 - 100 - 100 - 100 - 10100803765
AclW I
GGATC(N)4
CCTAG(N)5
Y+75 - 10050 - 750 - 100 - 10100303765
Aco I
YGGCCR
RCCGGY
G-50 - 7510050 - 7525 - 5075 - 1001003765
Acs I
RAATTY
YTTAAR
W+25 - 5050 - 7550 - 7510010 - 251005080
Acu I
CTGAAG(N)16
GACTTC(N)14
Y+ SAM+25 - 5050 - 7550 - 7575 - 100100503765
Afe I
AGCGCT
TCGCGA
Y-10 - 2525 - 5075 - 10075 - 1001001003765
Ags I
TTSAA
AASTT
Y+75 - 10050 - 7510 - 2510 - 25100503765
Ahl I
ACTAGT
TGATCA
B+10075 - 10025 - 5025 - 5075 - 10010037N
Ajn I
CCWGG
GGWCC
Y-25 - 5010 - 2510 - 2525 - 50100505565
Alu I
AGCT
TCGA
Y-75 - 10075 - 10010 - 2550 - 75100503765
AluB I
AGCT
TCGA
B+10075 - 10010 - 2510 - 2575 - 100603765
Ama87 I
CYCGRG
GRGCYC
W+10 - 2550 - 7575 - 1001000 - 10253765
Apa I
GGGCCC
CCCGGG
Y+50 - 7525 - 500 - 100 - 10100503765
Ars I
(N)8GACNNNNNNTTYG(N)11
(N)13CTGNNNNNNAARC(N)6
Y+0 - 100 - 100 - 100 - 10100203065
AsiG I
ACCGGT
TGGCCA
O-10 - 2525 - 5010075 - 10010 - 25753765
AspA2 I
CCTAGG
GGATCC
W+10 - 2550 - 7575 - 10010075 - 100103780
AspLE I
GCGC
CGCG
O-10 - 2575 - 10010050 - 7525 - 501003780
AspS9 I
GGNCC
CCNGG
W-50 - 7550 - 7575 - 10010050 - 75753765
AsuC2 I
CCSGG
GGSCC
Y-75 - 10050 - 7510 - 2525 - 50100403765
AsuHP I
GGTGA(N)8
CCACT(N)7
O-10 - 2550 - 7510075 - 10025 - 501003765
AsuNH I
GCTAGC
CGATCG
Y+75 - 10050 - 750 - 100 - 10100253765
BamH I
GGATCC
CCTAGG
G+25 - 5010075 - 10075 - 10025 - 501003765
Bar I
(N)7GAAGNNNNNNTAC(N)12
(N)12CTTCNNNNNNATG(N)7
*-0 - 100 - 1025 - 5050 - 7510 - 25403765
Bbv12 I
GWGCWC
CWCGWG
O-0 - 1010 - 2510075 - 10010 - 25603780
Bgl I
GCCNNNNNGGC
CGGNNNNNCCG
2W-50 - 7550 - 750 - 1075 - 10025 - 501003765
Bgl II
AGATCT
TCTAGA
O-0 - 1010 - 2510025 - 5010 - 251003780
Bme18 I
GGWCC
CCWGG
O-10 - 2525 - 5010075 - 10010 - 25803765
Bmt I
GCTAGC
CGATCG
W-10 - 2550 - 7550 - 7510075 - 1001003765
Bmu I
ACTGGG(N)5
TGACCC(N)4
Y-75 - 10075 - 10025 - 5010 - 25100253765
BpmI
CTGGAG(N)16
GACCTC(N)14
W+25 - 5050 - 7575 - 10010050 - 751003765
Bpu10I
CCTNAGC
GGANTCG
K-10 - 2525 - 5050 - 7550 - 7525 - 501003780
Bpu14 I
TTCGAA
AAGCTT
G-50 - 7510025 - 5025 - 5075 - 1001003765
Bsa29 I
ATCGAT
TAGCTA
G+25 - 5010050 - 7550 - 7575 - 1001003765
Bsc4 I
CCNNNNNNNGG
GGNNNNNNNCC
W+75 - 10075 - 10050 - 7510025 - 50905580
Bse1 I
ACTGGN
TGACCN
Y-75 - 10075 - 10025 - 5010 - 251001006580
Bse118 I
RCCGGY
YGGCCR
O-0 - 1050 - 7510075 - 10025 - 501006580
Bse21 I
CCTNAGG
GGANTCC
Y-50 - 7550 - 7510 - 2525 - 50100403780
Bse3D I
GCAATGNN
CGTTACNN
G-10 - 2510025 - 5050 - 7575 - 1001006080
Bse8 I
GATNNNNATC
CTANNNNTAG
G-25 - 5010075 - 10075 - 10050 - 751006080
BseP I
GCGCGC
CGCGCG
G-50 - 7510075 - 10050 - 7550 - 751005065
BseX3 I
CGGCCG
GCCGGC
O-10 - 2525 - 5010050 - 7510 - 25255080
BslF I
GGGAC(N)10
CCCTG(N)14
Y+25 - 5025 - 5010 - 2525 - 50100503780
Bso31 I
GGTCTC(N)1
CCAGAG(N)5
O+25 - 5075 - 10010075 - 10025 - 50405580
Bsp13 I
TCCGGA
AGGCCT
2K-25 - 5050 - 7575 - 10050 - 750 - 1055065
Bsp1720 I
GCTNAGC
CGANTCG
G-50 - 7510050 - 7550 - 7575 - 100753780
Bsp19 I
CCATGG
GGTACC
2W+0 - 1010 - 2550 - 7575 - 10010 - 2553765
BspAC I
CCGC
GGCG
O+10 - 2525 - 5010075 - 10010 - 251003765
BspFN I
CGCG
GCGC
Y-50 - 7575 - 10075 - 10050 - 751001003765
BssEC I
CCNNGG
GGNNCC
Y-50 - 7550 - 7550 - 7575 - 1001001006080
BssNA I
GTATAC
CATATG
W+50 - 7550 - 7575 - 10010075 - 10010037N
BssT1 I
CCWWGG
GGWWCC
2K-10 - 2525 - 5025 - 5075 - 10010 - 251006080
Bst2B I
CTCGTG
GAGCAC
Y+75 - 10025 - 5010 - 2525 - 501001006080
Bst2U I
CCWGG
GGWCC
G+75 - 10010050 - 7550 - 7510 - 255060N
Bst4C I
ACNGT
TGNCA
Y-75 - 10075 - 10010 - 2525 - 50100506580
Bst6 I
CTCTTC(N)1
GAGAAG(N)4
Y+75 - 10075 - 10050 - 7575 - 1001001006580
BstAC I
GRCGYC
CYGCRG
W-75 - 10075 - 10050 - 7510075 - 1001003780
BstAF I
CTTAAG
GAATTC
W+10 - 2525 - 5075 - 10010025 - 501005580
BstAP I
GCANNNNNTGC
CGTNNNNNACG
W-25 - 5025 - 5075 - 10010025 - 501006080
BstAU I
TGTACA
ACATGT
W-10 - 2550 - 7525 - 5010025 - 501003780
BstBA I
YACGTR
RTGCAY
W+25 - 5025 - 5075 - 10010025 - 50506580
BstC8I
GCNNGC
CGNNCG
Y-10 - 2525 - 5050 - 7575 - 1001001005580
BstDE I
CTNAG
GANTC
G-75 - 10010025 - 5050 - 7510 - 251006080
BstDS I
CCRYGG
GGYRCC
Y-0 - 1075 - 10050 - 7525 - 501001006580
BstEN I
CCTNNNNNAGG
GGANNNNNTCC
Y-50 - 7550 - 7525 - 5025 - 501001006580
BstF5 I
GGATGNN
CCTACNN
Y-75 - 10050 - 7525 - 5050 - 751001006580
BstFN I
CGCG
GCGC
Y-75 - 10050 - 7525 - 5025 - 50100756080
BstH2 I
RGCGCY
YCGCGR
Y+50 - 7550 - 750 - 1010 - 251001006580
BstHH I
GCGC
CGCG
Y+75 - 10050 - 7525 - 5050 - 7510010050N
BstKTI
GATC
CTAG
W-25 - 5050 - 7575 - 10010050 - 751003765
BstMA I
GTCTC(N)1
CAGAG(N)5
W+25 - 5050 - 7550 - 7510075 - 1001005565
BstMB I
GATC
CTAG
O-10 - 2525 - 5010075 - 10010 - 251006580
BstMC I
CGRYCG
GCYRGC
B+10075 - 10010 - 2510 - 2550 - 751005080
BstMW I
GCNNNNNNNGC
CGNNNNNNNCG
Y-10 - 2525 - 5025 - 5050 - 75100405580
BstNS I
RCATGY
YGTACR
B+10050 - 7510 - 2510 - 2575 - 100503765
BstPA I
GACNNNNGTC
CTGNNNNCAG
Y-50 - 7525 - 5050 - 7550 - 7510010065N
BstSCI
CCNGG
GGNCC
Y-50 - 7550 - 7550 - 7550 - 75100605580
BstSF I
CTRYAG
GAYRTC
O+75 - 10025 - 5010050 - 7550 - 7510060N
BstSL I
GKGCMC
CMCGKG
G+50 - 7510050 - 7575 - 10075 - 1001005565
BstSN I
TACGTA
ATGCAT
B-10050 - 750 - 1010 - 2550 - 75503780
BstV1I
GCAGC(N)8
CGTCG(N)12
G-75 - 10010075 - 10075 - 10075 - 1001005580
BstV2 I
GAAGAC(N)2
CTTCTG(N)6
Y+75 - 10075 - 10025 - 5025 - 50100705565
BstX I
CCANNNNNNTGG
GGTNNNNNNACC
O-10 - 2510 - 2510075 - 10025 - 501003765
BstX2 I
RGATCY
YCTAGR
G-75 - 1001000 - 1010 - 2525 - 501006080
Bsu I
GTATCC(N)6
CATAGG(N)5
Y-75 - 10050 - 7510 - 2525 - 50100103765
BsuR I
GGCC
CCGG
G-75 - 10010025 - 5050 - 7550 - 751003780
Btr I
CACGTC
GTGCAG
O+75 - 10075 - 10010075 - 10075 - 1001006080
Cci I
TCATGA
AGTACT
W+0 - 1010 - 2525 - 5010075 - 1001005580
CciN I
GCGGCCGC
CGCCGGCG
Y-25 - 5050 - 7575 - 10075 - 1001001003765
Dra I
TTTAAA
AAATTT
G+75 - 10010025 - 5075 - 10075 - 1001003765
DraIII
CACNNNGTG
GTGNNNCAC
2K+25 - 5050 - 7575 - 10075 - 10050 - 751003765
Dri I
GACNNNNNGTC
CTGNNNNNCAG
Y-75 - 10075 - 10010 - 2510 - 25100403765
DseD I
GACNNNNNNGTC
CTGNNNNNNCAG
Y+75 - 10075 - 10025 - 5050 - 75100303780
EcoICR I
GAGCTC
CTCGAG
G+75 - 1001000 - 100 - 1075 - 10053765
EcoR I
GAATTC
CTTAAG
*+50 - 7575 - 10075 - 10075 - 10050 - 75503765
EcoR V
GATATC
CTATAG
W+0 - 1025 - 5050 - 7510025 - 50503780
Ege I
GGCGCC
CCGCGG
B+10075 - 10010 - 2550 - 7575 - 1001003765
Erh I
CCWWGG
GGWWCC
2W+10 - 2525 - 5075 - 10075 - 10010 - 251003765
FaeI
CATG
GTAC
*+25 - 5050 - 7510 - 2510 - 2575 - 1001003765
FaiI
YATR
RTAY
B-10050 - 7510 - 2525 - 5025 - 501005080
FalI
(N)8AAGNNNNNCTT(N)13
(N)13TTCNNNNNGAA(N)8
W+ SAM-0 - 1025 - 5075 - 10010050 - 75703765
FatI
CATG
GTAC
G-10 - 2510025 - 5010 - 2550 - 751005565
Fau I
CCCGC(N)4
GGGCG(N)6
B-10025 - 500 - 100 - 1050 - 75505565
FauND I
CATATG
GTATAC
Y+50 - 7575 - 10010 - 2550 - 751001003765
Fbl I
GTMKAC
CAKMTG
Y-50 - 7575 - 1000 - 1050 - 751001005580
Fok I
GGATG(N)9
CCTAC(N)13
Y-50 - 7550 - 7525 - 5025 - 501001003765
FriO I
GRGCYC
CYCGRG
Y+75 - 10075 - 10010 - 250 - 10100253765
Fsp4H I
GCNGC
CGNCG
Y-50 - 7575 - 10010 - 2525 - 501001003765
Gsa I
CCCAGC
GGGTCG
W+10 - 2525 - 5075 - 10010075 - 10010070N
Hae III
GGCC
CCGG
G-75 - 10010025 - 5050 - 7550 - 751003780
Hga I
GACGC(N)5
CTGCG(N)10
B-10075 - 10010 - 2525 - 5050 - 75503765
Hind II
GTYRAC
CARYTG
G+75 - 10010025 - 5025 - 5075 - 10053765
Hind III
AAGCTT
TTCGAA
W+10 - 2525 - 500 - 101000 - 101003780
Hinf I
GANTC
CTNAG
O-25 - 5075 - 10010075 - 10075 - 1001003780
Hpa I
GTTAAC
CAATTG
Y-0 - 1050 - 7510 - 2525 - 50100253765
Hpa II
CCGG
GGCC
B-10050 - 7510 - 2525 - 5050 - 75503780
HpySE526 I
ACGT
TGCA
Y-75 - 10075 - 10010 - 2525 - 50100503765
HspA I
GCGC
CGCG
Y-50 - 7550 - 7525 - 5025 - 501001003780
Kpn I
GGTACC
CCATGG
B+10025 - 5025 - 5025 - 5075 - 100503780
Ksp22 I
TGATCA
ACTAGT
2K+50 - 7575 - 10050 - 7550 - 7525 - 501003765
Kzo9 I
GATC
CTAG
G-50 - 7510050 - 7550 - 7550 - 751003765
Lmn I
GCTCCN
CGAGGN
B-10075 - 10050 - 7550 - 7575 - 100803765
Mab I
ACCWGGT
TGGWCCA
W+25 - 5050 - 7575 - 10010050 - 751003765
Mbo II
GAAGA(N)8
CTTCT(N)7
Y-75 - 10075 - 10025 - 5050 - 75100503765
Mhl I
GDGCHC
CHCGDG
W-10 - 2525 - 5075 - 10010010 - 251003780
Mlu I
ACGCGT
TGCGCA
O-0 - 1010 - 2510025 - 5010 - 25503765
Mly113 I
GGCGCC
CCGCGG
B-10025 - 5010 - 2510 - 2550 - 751003765
Mnl I
CCTC(N)7
GGAG(N)6
G+75 - 10010025 - 5025 - 5075 - 1001003765
Mox20 I
TGGCCA
ACCGGT
O-10 - 2525 - 5010075 - 10025 - 507537N
MroN I
GCCGGC
CGGCCG
B-10050 - 7510 - 250 - 1010 - 2553780
MroX I
GAANNNNTTC
CTTNNNNAAG
W-50 - 7550 - 7550 - 7510025 - 501003765
Msp I
CCGG
GGCC
B-10075 - 10050 - 7575 - 10075 - 1001003765
MspA1 I
CMGCKG
GKCGMC
Y+10 - 2575 - 10010 - 2525 - 501001003765
MspR9 I
CCNGG
GGNCC
O-50 - 7550 - 7510050 - 7575 - 1001003780
Nru I
TCGCGA
AGCGCT
W-0 - 1010 - 2575 - 10010010 - 251003780
PalA I
GGCGCGCC
CCGCGCGG
Y-25 - 5010 - 250 - 100 - 10100403765
Pce I
AGGCCT
TCCGGA
Y-75 - 10075 - 10050 - 7525 - 501001005080
Pci I
ACATGT
TGTACA
O-50 - 7575 - 10010075 - 10050 - 75503765
PciS I
GCTCTTCN
CGAGAAG(N)4
B-10050 - 750 - 100 - 1075 - 100503765
Pct I
GAATGCN
CTTACGN
O-25 - 5050 - 7510075 - 10010 - 25503765
Ple19 I
CGATCG
GCTAGC
Y-75 - 10075 - 10025 - 5025 - 501001003765
Pps I
GAGTC(N)4
CTCAG(N)5
Y+50 - 7510 - 250 - 1025 - 50100503765
Psi I
TTATAA
AATATT
B-10025 - 5010 - 2525 - 5075 - 100403765
Psp124B I
GAGCTC
CTCGAG
G-75 - 10010010 - 250 - 1075 - 100303780
Psp6 I
CCWGG
GGWCC
B-10050 - 7510 - 2525 - 5075 - 100105580
PspC I
CACGTG
GTGCAC
B+10050 - 750 - 100 - 1050 - 7553765
PspE I
GGTNACC
CCANTGG
B-10050 - 7525 - 5050 - 7550 - 751003765
PspL I
CGTACG
GCATGC
Y+75 - 10075 - 10025 - 5010 - 251001003765
PspN4 I
GGNNCC
CCNNGG
Y-10 - 2510 - 2510 - 2525 - 50100503765
PspOM I
GGGCCC
CCCGGG
Y-75 - 10010 - 250 - 100 - 10100253765
PspPP I
RGGWCCY
YCCWGGR
Y-10 - 2510 - 250 - 100 - 101001003765
PspX I
VCTCGAGB
BGAGCTCV
Y+50 - 7550 - 7525 - 5075 - 100100253780
Psr I
(N)7GAACNNNNNNTAC(N)12
(N)12CTTGNNNNNNATG(N)7
Y+10 - 2510 - 250 - 100 - 10100303065
Pst I
CTGCAG
GACGTC
O+10 - 2525 - 5010025 - 5025 - 50503780
PstN I
CAGNNNCTG
GTCNNNGAC
Y-25 - 5050 - 7510 - 2525 - 501001003765
Pvu II
CAGCTG
GTCGAC
G+25 - 5010025 - 5025 - 5025 - 501003780
Rga I
GCGATCGC
CGCTAGCG
Y-75 - 10050 - 7510 - 2525 - 501001005580
Rig I
GGCCGGCC
CCGGCCGG
*+75 - 10050 - 750 - 1010 - 2550 - 75103765
Rsa I
GTAC
CATG
B-10050 - 750 - 1050 - 7575 - 1005037N
RsaN I
GTAC
CATG
B-10075 - 10050 - 7550 - 7575 - 1001003780
Rsr2 I
CGGWCCG
GCCWGGC
Y+50 - 7575 - 1000 - 1010 - 25100253765
Sal I
GTCGAC
CAGCTG
O-0 - 1010 - 2510025 - 500 - 1053765
Sbf I
CCTGCAGG
GGACGTCC
Y-75 - 10050 - 750 - 100 - 10100503780
Set I
ASST
TSSA
Y-25 - 5025 - 5075 - 10075 - 1001001005580
SfaN I
GCATC(N)5
CGTAG(N)9
O-10 - 2525 - 5010075 - 1000 - 10253780
Sfi I
GGCCNNNNNGGCC
CCGGNNNNNCCGG
G+75 - 10010025 - 5025 - 5025 - 50755065
Sfr274 I
CTCGAG
GAGCTC
B-10075 - 10050 - 7550 - 7575 - 1001005065
Sfr303 I
CCGCGG
GGCGCC
B-10050 - 7510 - 2510 - 2575 - 1001003765
Sma I
CCCGGG
GGGCCC
Y-0 - 100 - 100 - 100 - 10100502565
Smi I
ATTTAAAT
TAAATTTA
O+25 - 5025 - 5010075 - 10025 - 50103765
SmiM I
CAYNNNNRTG
GTRNNNNYAC
W-10 - 2510 - 2575 - 10010010 - 25603765
Sph I
GCATGC
CGTACG
G+25 - 5010075 - 10075 - 10050 - 751003765
Sse9 I
AATT
TTAA
B+10075 - 10050 - 7550 - 7575 - 100755565
Ssp I
AATATT
TTATAA
K+75 - 10050 - 7525 - 5050 - 7575 - 1001003765
SspM I
CTAG
GATC
Y-50 - 7525 - 5010 - 2550 - 751008055N
Taq I
TCGA
AGCT
Y+50 - 7575 - 10075 - 10050 - 751001006580
Tru9 I
TTAA
AATT
W-75 - 10025 - 5025 - 5010050 - 751006580
TseF I
GTSAC
CASTG
B-10050 - 750 - 1025 - 5050 - 755065N
Tth111 I
GACNNNGTC
CTGNNNCAG
Y-75 - 10050 - 7510 - 2510 - 251001006580
Vne I
GTGCAC
CACGTG
O-10 - 2525 - 5010025 - 5025 - 501003765
Vsp I
ATTAAT
TAATTA
W-0 - 1010 - 2550 - 7510025 - 50503765
Xba I
TCTAGA
AGATCT
O+75 - 10075 - 10010050 - 7575 - 100253765
Xma I
CCCGGG
GGGCCC
Y-75 - 10050 - 750 - 100 - 10100503765
ZraI
GACGTC
CTGCAG
B-10050 - 7525 - 5025 - 5075 - 1001003780
Zrm I
AGTACT
TCATGA
Y+50 - 7525 - 500 - 100 - 10100503765
Zsp2 I
ATGCAT
TACGTA
B+10050 - 7525 - 5025 - 5025 - 50503765
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