Блокирование активности рестриктаз при полном или частичном перекрывании их сайтов узнавания метилированием

 

Рестриктаза

Позиция гидролиза

Прототип

Блокирование
рестриктазы при полном или частичном перекрывании её сайта узнавания
метилированием

сопряжённой ДНК
метилазой

dam
Gm6ATC

dcm
Cm5CWGG

M.SssI
m5CG

AatII

GACGT↑C
C↓TGCAG

AatII

G(m6A)CGTC

AbsI

CC↑TCGAGG
GGAGCT↓CC

AbsI

Acc16I

TGC↑GCA
ACG↓CGT

MstI

Acc36I

ACCTGC(N)4↑
TGGACG(N)8↓

BspMI

Acc65I

G↑GTACC
CCATG↓G

KpnI

+

AccB1I

G↑GYRCC
CCRYG↓G

HgiCI

AccB7I

CCANNNN↑NTGG
GGTN↓NNNNACC

PflMI

+

AccBSI

GAG↑CGG
CTC↓GCC

BsrBI

AclI

AA↑CGTT
TTGC↓AA

AclI

+

AclWI

GGATC(N)4↑
CCTAG(N)5↓

BinI

+

AcoI

Y↑GGCCR
RCCGG↓Y

CfrI

+

AcsI

R↑AATTY
YTTAA↓R

ApoI

AcuI

CTGAAG(N)16↑
GACTTC(N)14↓

Eco57I

AfeI

AGC↑GCT
TCG↓CGA

Eco47III

AgsI

TTS↑AA
AA↓STT

AgsI

TTSA(m6A)

AhlI

A↑CTAGT
TGATC↓A

SpeI

AjnI

↑CCWGG
GGWCC↓

EcoRII

(m5C)CWGG

AluI

AG↑CT
TC↓GA

AluI

AG(m5C)T

AluBI

AG↑CT
TC↓GA

AluI

(m6A)GCT

Ama87I

C↑YCGRG
GRGCY↓C

AvaI

AoxI

↑RG(5mC)Y
Y(5mC)GR↓

AoxI

ApaI

GGGCC↑C
C↓CCGGG

ApaI

+

ArsI

↑(N)8GACNNNNNNTTYG(N)11↑
↓(N)13CTGNNNNNNAARC(N)6↓

ArsI

AsiGI

A↑CCGGT
TGGCC↓A

AgeI

AsiSI

GCGAT↑CGC
CGC↓TAGCG

SgfI

+

AspA2I

C↑CTAGG
GGATC↓C

AvrII

AspLEI

GCG↑C
C↓GCG

HhaI

+

AspS9I

G↑GNCC
CCNG↓G

Sau96I

+

AsuC2I

CC↑SGG
GGS↓CC

CauII

AsuHPI

GGTGA(N)8↑
CCACT(N)7↓

HphI

+

AsuNHI

G↑CTAGC
CGATC↓G

NheI

BamHI

G↑GATCC
CCTAG↓G

BamHI

GGAT(m4C)C

BarI

↑(N)7GAAGNNNNNNTAC(N)12↑
↓(N)12CTTCNNNNNNATG(N)7↓

BarI

Bbv12I

GWGCW↑C
C↓WCGWG

HgiAI

BglI

GCCNNNN↑NGGC
CGGN↓NNNNCCG

BglI

BglII

A↑GATCT
TCTAG↓A

BglII

BlsI

Последовательность
ДНК,
содержащая не менее двух 5mC:
RYN↑RY
RYN↓RY

BlsI

Bme18I

G↑GWCC
CCWG↓G

AvaII

+

BmtI

GCTAG↑C
C↓GATCG

NheI

BmuI

ACTGGG(N)5↑
TGACCC(N)4↓

BfiI

BpmI

CTGGAG(N)16↑
GACCTC(N)14↓

GsuI

Bpu10I

CC↑TNAGC
GGANT↓CG

Bpu10I

Bpu14I

TT↑CGAA
AAGC↓TT

AsuII

Bsa29I

AT↑CGAT
TAGC↓TA

ClaI

+

Bsc4I

CCNNNNN↑NNGG
GGNN↓NNNNNCC

BsiYI

Bse1I

ACTGGN↑
TGAC↓CN

BsrI

Bse118I

R↑CCGGY
YGGCC↓R

Cfr10I

Bse21I

CC↑TNAGG
GGANT↓CC

SauI

Bse3DI

GCAATGNN↑
CGTTAC↓NN

BsrDI

Bse8I

GATNN↑NNATC
CTANN↓NNTAG

BsaBI

BsePI

G↑CGCGC
CGCGC↓G

BsePI

BseX3I

C↑GGCCG
GCCGG↓C

XmaIII

BslFI

GGGAC(N)10↑
CCCTG(N)14↓

FinI

Bso31I

GGTCTCN↑
CCAGAG(N)5↓

Eco31I

Bsp13I

T↑CCGGA
AGGCC↓T

BspMII

+

Bsp1720I

GC↑TNAGC
CGANT↓CG

EspI

Bsp19I

C↑CATGG
GGTAC↓C

NcoI

(m4C)CATGG
(m5C)CATGG/GGTAC(m5C)

BspACI

C↑CGC
GGC↓G

AciI

+

BspFNI

CG↑CG
GC↓GC

FnuDII

+

BssECI

C↑CNNGG
GGNNC↓C

SecI

(m4C)CNNGG

BssNAI

GTA↑TAC
CAT↓ATG

SnaI

BssT1I

C↑CWWGG
GGWWC↓C

StyI

Bst2BI

C↑TCGTG
GAGCA↓C

BsiI

Bst2UI

CC↑WGG
GGW↓CC

BstNI

Bst4CI

ACN↑GT
TG↓NCA

Tsp4CI

Bst6I

CTCTTCN↑
GAGAAG(N)4↓

Ksp632I

BstACI

GR↑CGYC
CYGC↓RG

AcyI

BstAFI

C↑TTAAG
GAATT↓C

AflII

BstAPI

GCANNNN↑NTGC
CGTN↓NNNNACG

(ApaBI)

BstAUI

T↑GTACA
ACATG↓T

Bsp1407I

BstBAI

YAC↑GTR
RTG↓CAY

BsaAI

+

BstC8I

GCN↑NGC
CGN↓NCG

Cac8I

G(m5C)NNGC

BstDEI

C↑TNAG
GANT↓C

DdeI

BstDSI

C↑CRYGG
GGYRC↓C

DsaI

BstENI

CCTNN↑NNNAGG
GGANNN↓NNTCC

EcoNI

BstF5I

GGATGNN↑
CCTAC↓NN

(FokI)

BstFNI

CG↑CG
GC↓GC

FnuDII

+

BstH2I

RGCGC↑Y
Y↓CGCGR

HaeII

BstHHI

GCG↑C
C↓GCG

HhaI

+

BstKTI

GAT↑C
C↓TAG

MboI

+

BstMAI

GTCTCN↑
CAGAG(N)5↓

BsmAI

BstMBI

↑GATC
CTAG↓

MboI

+

BstMCI

CGRY↑CG
GC↓YRGC

McrI

BstMWI

GCNNNNN↑NNGC
CGNN↓NNNNNCG

MwoI

G(m5C)NNNNNNNGC

BstNSI

RCATG↑Y
Y↓GTACR

NspI

BstPAI

GACNN↑NNGTC
CTGNN↓NNCAG

PshAI

BstSCI

↑CCNGG
GGNCC↓

ScrFI

+

BstSFI

C↑TRYAG
GAYRT↓C

SfeI

BstSLI

GKGCM↑C
C↓MCGKG

BseSI

GKG(m5C)MC

BstSNI

TAC↑GTA
ATG↓CAT

SnaBI

BstV1I

GCAGC(N)8↑
CGTCG(N)12↓

BbvI

BstV2I

GAAGAC(N)2↑
CTTCTG(N)6↓

BbvII

BstXI

CCANNNNN↑NTGG
GGTN↓NNNNNACC

BstXI

BstX2I

R↑GATCY
YCTAG↓R

XhoII

BstX2I

R↑GATCY
YCTAG↓R

XhoII

BsuI

GTATCC(N)6↑
CATAGG(N)5↓

BciVI

BsuRI

GG↑CC
CC↓GG

HaeIII

BtrI

CAC↑GTC
GTG↓CAG

BtrI

CciI

T↑CATGA
AGTAC↓T

BspHI

CciNI

GC↑GGCCGC
CGCCGG↓CG

NotI

+

DraI

TTT↑AAA
AAA↓TTT

AhaIII

DraIII

CACNNN↑GTG
GTG↓NNNCAC

DraIII

DriI

GACNNN↑NNGTC
CTGNN↓NNNCAG

Eam1105I

DseDI

GACNNNN↑NNGTC
CTGNN↓NNNNCAG

DrdI

EcoICRI

GAG↑CTC
CTC↓GAG

SacI

EcoRI

G↑AATTC
CTTAA↓G

EcoRI

GA(m6A)TTC

EcoRV

GAT↑ATC
CTA↓TAG

EcoRV

EgeI

GGC↑GCC
CCG↓CGG

NarI

ErhI

C↑CWWGG
GGWWC↓C

StyI

FaeI

CATG↑
↓GTAC

NlaIII

C(m6A)TG

FaiI

YA↑TR
RT↓AY

FaiI

FalI

↑(N)8AAGNNNNNCTT(N)13↑
↓(N)13TTCNNNNNGAA(N)8↓

FalI

FatI

↑CATG
GTAC↓

NlaIII

(m5C)ATG

FauI

CCCGC(N)4↑
GGGCG(N)6↓

FauI

+

FauNDI

CA↑TATG
GTAT↓AC

NdeI

FblI

GT↑MKAC
CAKM↓TG

AccI

FokI

GGATG(N)9↑
CCTAC(N)13↓

FokI

FriOI

GRGCY↑C
C↓YCGRG

BanII

Fsp4HI

GC↑NGC
CGN↓CG

Fnu4HI

G(m5C)NGC

GlaI

R(5mC)↑GY
YG↓(5mC)R

GlaI

GluI

G(5mC)↑NG(5mC)
(5mC)GN↓(5mC)G

GluI

GsaI

CCCAG↑C
G↓GGTCG

BseYI

HaeIII

GG↑CC
CC↓GG

HaeIII

GG(m5C)C

HgaI

GACGC(N)5↑
CTGCG(N)10↓

HgaI

+

HindII

GTY↑RAC
CAR↓YTG

HindII

HindIII

A↑AGCTT
TTCGA↓A

HindIII

HinfI

G↑ANTC
CTNA↓G

HinfI

HpaI

GTT↑AAC
CAA↓TTG

HpaI

+

HpaII

C↑CGG
GGC↓C

HpaII

C(m5C)GG, а так же
MspI-метилированием:
(m5C)CGG

+

HpySE526I

A↑CGT
TGC↓A

MaeII

HspAI

G↑CGC
CGC↓G

HhaI

+

KpnI

GGTAC↑C
C↓CATGG

KpnI

KroI

G↑C(5mC)GGC
CGG(5mC)C↓G

KroI

KroNI

GCC↑GGC
CGG↓CCG

NaeI

+

Ksp22I

T↑GATCA
ACTAG↓T

BclI

+

Kzo9I

↑GATC
CTAG↓

MboI

+
GAT(m5C)G

LmnI

GCTCCN↑
CGAG↓GN

LmnI

MabI

A↑CCWGGT
TGGWCC↓A

SexAI

MalI

G(mA)↑TC
CT↓(mA)G

DpnI

MboII

GAAGA(N)8↑
CTTCT(N)7↓

MboII

+

MfeI

C↑AATTG
GTTAA↓C

MfeI

MhlI

GDGCH↑C
C↓HCGDG

SduI

MluI

A↑CGCGT
TGCGC↓A

MluI

Mly113I

GG↑CGCC
CCGC↓GG

NarI

MnlI

CCTC(N)7↑
GGAG(N)6↓

MnlI

+
CCT(m5C)G

Mox20I

TGG↑CCA
ACC↓GGT

BalI

+

MroNI

G↑CCGGC
CGGCC↓G

(NaeI)

MroXI

GAANN↑NNTTC
CTTNN↓NNAAG

XmnI

MspI

C↑CGG
GGC↓C

HpaII

(m5C)CGG

MspA1I

CMG↑CKG
GKC↓GMC

NspBII

MspR9I

CC↑NGG
GGN↓CC

ScrFI

+

MteI

G(5mC)G(5mC)↑NG(5mC)G(5mC)
(5mC)G(5mC)GN↓(5mC)G(5mC)G

Mte

NruI

TCG↑CGA
AGC↓GCT

NruI

+

PalAI

GG↑CGCGCC
CCGCGC↓GG

AscI

+

PceI

AGG↑CCT
TCC↓GGA

StuI

PciI

A↑CATGT
TGTAC↓A

BspLU11I

(m6A)CATGT

PciSI

GCTCTTCN↑
CGAGAAG(N)4↓

SapI

PcsI

(5mC)GNNNNN↑NN(5mC)G
G(5mC)NN↓NNNNNG(5mC)

PcsI

PctI

GAATGCN↑
CTTAC↓GN

BsmI

PkrI

Последовательность
ДНК,
содержащая не менее трех 5mC:
GCN↑GC
CG↓NCG

PkrI

Ple19I

CGAT↑CG
GC↓TAGC

PvuI

PpsI

GAGTC(N)4↑
CTCAG(N)5↓

PleI

PsiI

TTA↑TAA
AAT↓ATT

PsiI

Psp124BI

GAGCT↑C
C↓TCGAG

SacI

Psp6I

↑CCWGG
GGWCC↓

EcoRII

+

PspCI

CAC↑GTG
GTG↓CAC

PmaCI

PspEI

G↑GTNACC
CCANTG↓G

BstEII

PspLI

C↑GTACG
GCATG↓C

SplI

PspN4I

GGN↑NCC
CCN↓NGG

NlaIV

GGNN(m5C)C

PspOMI

G↑GGCCC
CCCGG↓G

(ApaI)

PspPPI

RG↑GWCCY
YCCWG↓GR

PpuMI

+

PspXI

VC↑TCGAGB
BGAGCT↓CV

PspXI

PsrI

↑(N)7GAACNNNNNNTAC(N)12↑
↓(N)12CTTGNNNNNNATG(N)7↓

PsrI

PstI

CTGCA↑G
G↓ACGTC

PstI

PstNI

CAGNNN↑CTG
GTC↓NNNGAC

AlwNI

PvuII

CAG↑CTG
GTC↓GAC

PvuII

RgaI

GCGAT↑CGC
CGC↓TAGCG

SgfI

RigI

GGCCGG↑CC
CC↓GGCCGG

FseI

GG(m5C)CGGCC

+

RsaI

GT↑AC
CA↓TG

RsaI

RsaNI

G↑TAC
CAT↓G

RsaI

Rsr2I

CG↑GWCCG
GCCWG↓GC

RsrII

SalI

G↑TCGAC
CAGCT↓G

SalI

SbfI

CCTGCA↑GG
GG↓ACGTCC

Sse8387I

SetI

ASST↑
↓TSSA

SetI

SfaNI

GCATC(N)5↑
CGTAG(N)9↓

SfaNI

SfiI

GGCCNNNN↑NGGCC
CCGGN↓NNNNCCGG

SfiI

+
GGC(m5C)WGGNNGGCC

Sfr274I

C↑TCGAG
GAGCT↓C

XhoI

CTCG(m6A)G

Sfr303I

CCGC↑GG
GG↓CGCC

SacII

SmaI

CCC↑GGG
GGG↓CCC

SmaI

SmiI

ATTT↑AAAT
TAAA↓TTTA

SwaI

SmiMI

CAYNN↑NNRTG
GTRNN↓NNYAC

MslI

SphI

GCATG↑C
C↓GTACG

SphI

Sse9I

↑AATT
TTAA↓

Tsp509I

A(m6A)TT

SspI

AAT↑ATT
TTA↓TAA

SspI

A(m6A)TATT

TaqI

T↑CGA
AGC↓T

TaqI

Tru9I

T↑TAA
AAT↓T

MseI

TTA(m6A)

TseFI

↑GTSAC
CASTG↓

Tsp45I

Tth111I

GACN↑NNGTC
CTGNN↓NCAG

Tth111I

VneI

G↑TGCAC
CACGT↓G

ApaLI

VspI

AT↑TAAT
TAAT↓TA

VspI

ATTA(m6A)T

XbaI

T↑CTAGA
AGATC↓T

XbaI

+

XmaI

C↑CCGGG
GGGCC↓C

XmaI

ZraI

GAC↑GTC
CTG↓CAG

(AatII)

ZrmI

AGT↑ACT
TCA↓TGA

ScaI

Zsp2I

ATGCA↑T
T↓ACGTA

NsiI