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Enzymatic properties


VA Chernukhin, DA Gonchar, MA Abdurashitov, EV Kileva, OA Belichenko, VS Dedkov, NA Mikhnenkova, SKh Degtyarev Comparative analysis of biochemical properties of site specific methyl-directed DNA endonucleases BisI, BlsI and EcoBLI recognizing 5`-G(5mC)NGC-3`/3`-CGN(5mC)G-5` // Research Journal of Pharmaceutical, Biological and Chemical Sciences, RJPBCS, vol 7(2), p.p. 388-394 (2016)
Murat A . Abdurashitov , Valery A . Chernukhin , Danila A . Gonchar , Vladimir S . Dedkov, Natalia A . Mikhnenkova, and Sergey Kh . Degtyarev Dimethyl Sulfoxide Changes the Recognition Site Preference of Methyl- directed Site-specific DNA Endonuclease GlaI.// Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 7(1),p. 1733-1739 (2016)
Zemlyanskaya, E.V., Degtyarev, S.K. Substrate specificity and properties of methyl-directed site-specific DNA endonucleases // Molecular Biology, v.47, No.6, p.900-913 (2013)
V.A. Chernukhin, D.A. Gonchar, Yu.E. Tomilova, A.A. Boltengagen, L.N. Golikova, S.Kh. Degtyarev Methyl-directed site-specific endonuclease BlsI recognizes and cleaves DNA sequence 5Т-RYNRY-3Т carrying of at least two 5-methylcytosines // Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov (Moscow), V.9, No.2, p.30-38 (2013). (In Russian)
Dedkov V.S., Mikhnenkova N.A., Djanobilova Z.K., Tarasova M.V. A M.BssECI DNA Methyltransferase Forms 5`-m4CCNNGG-3`. Sensitivity of Restriction Endonucleases to the New Methylation. // Biotekhnologia (Moscow), No 2, p.32-42 (2012). (In Russian)
Tarasova, M.V., Kuznetsov, V.V., Netesova, N.A., Gonchar, D.A., Degtyarev, S.K. Cloning and analysis of biochemical and catalytic properties of DNA methyltransferase M1.BspACI // Moscow University Biological Sciences Bulletin, Series 16: Biology, No.2, p.38-40 (2011)
Ju.E. Tomilova, V.A. Chernukhin, S.Kh. Degtyarev A new site-specific DNA endonuclease FaiI recognizes and cuts tetranucleotide sequence 5`-YA^TR-3`.// Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov (Moscow), V.7, No.2, p.11-16 (2011). (In Russian)
Tomilova J.E., Kuznetsov V.V., Abdurashitov M.A., Netesova N.A., Degtyarev S.Kh. Recombinant DNA-methyltransferase M1.Bst19I from Bacillus stearothermophilus 19: purification, properties and amino acids sequence analysis.// Molecular Biology(Moscow), Vol.44, No. 4, 688-698 (2010). (In Russian)
Chernukhin V.A., Kuznetsov V.V., Gonchar D.A., Kashirina Yu.G., Netesova N.A., Degtyarev S. Kh. Analysis of substrate specificity and biochemical properties of DNA methyltransferase M3.BstF5I from BstF5I restriction-modification system // Biochemistry (Moscow), Vol. 75, No 1, p.78-87 (2010). (In Russian)
Dedkov V.S. Analysis of Specificity of DNA-Methyltransferase M.AspS9I in Cell Lysate by means of Restriction Endonucleases Blocking. // Biotekhnologia (Moscow), No 4, p.30-39 (2009). (In Russian)
Dedkov V.S. Determination of Specificity of DNA Methyltransferase M.Bsc4I in Cellular Lysate by Blocking Restriction Endonucleases and Computer Modeling. // Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya (Moscow), No 3, p.3-8 (2009). (In Russian)
Chernukhin V.A., Seggewiss J., Kashirina Yu.G., Gonchar D.A., Degtyarev S.Kh. Purification of recombinant DNA methyltransferase M2.BstSEI from nickase-modification system NM.BstSEI and study of the enzyme properties. // Molecular Biology(Moscow), Vol.43, No. 1, 10-18 (2009). (In Russian)
Tarasova G. V., Nayakshina T. N., Degtyarev S. Kh. Substrate specificity of new methyl-directed DNA endonuclease GlaI . // BMC Molecular Biology 2008, 9:7
Tomilova J.E., Chernukhin V.A., Degtyarev S.Kh. Dependence of site-specific endonuclease GlaI activity on quantity and location of methylcytosines in the recognition sequence 5`-GCGC-3`. // Bulletin of biotechnology and physico-chemical biology V.2, No 1, p. 30-39 (2006) (In Russian)
Chernukhin V.A., Kashirina Yu.G., Sukhanova K.S., Abdurashitov M.A., Gonchar D.A., and Degtyarev S. Kh. Isolation and exploration of biochemical propeties of DNA Methyltransferase FauIA modifying Non-palindromic sequence 5'-CCCGC-3'. // Biochemistry (Moscow), Vol. 70, No 6, (2005). (In Russian)
Akishev A.G., Rechkunova N. I., Tomilova J. E., Lebedeva N. A., Lavrik O. I., and Degtyarev S. Kh. Thermostable DNA polymerase from Thermus thermophilus B35: preparation and study of a modified form of the enzyme with high affinity to ddNTP. // Biochemistry (Moscow), Vol. 68, No 12, (2003). (In Russian)
Chernukhin V. A., Golikova L. N., Gonchar D. A., Abdurashitov M. A., Kashirina Yu. G., Netesova N. A., and Degtyarev S. Kh. M.BstF5I-2 and M.BstF5I-4 DNA Methyltransferases from BstF5I restriction-modification system of Bacillus stearothermophilus F5. // Biochemistry (Moscow), Vol. 68, No 9, (2003). (In Russian)
N.A.Netesova, L.N.Golikova, L.G. Ovechkina, A.A. Evdokimov, E.G. Malygin, N.S. Gololobova, D.A. Gonchar and S.Kh.Degtyarev. The restriction-modification system from Bacillus stearothermophilius F5: Comparison study of the DNA methyltransferases M.BstF5I-1 and M.BstF5I-3. // Molecular Biology(Moscow), vol.36, p.1-8 (2002). (In Russian)
Rechkunova N. I., Okhapkina S. S., Anarbaev R. O., Lokhova I. A., Degtyarev S. Kh., and Lavrik O. I. Thermostable DNA polymerase from Thermus thermophilus B35: Influence of divalent metal ions on the interaction with deoxynucleoside triphosphates. // Biochemistry (Moscow), Vol. 65, No 5 (2000). (In Russian)
Zakharenko A.L., Khodyreva S.N., Rechkunova N.I., Safronov I.V., Pyshnyi D.V., Degtyarev S.Kh., Lavrik O.I. Photoaffinity labeling of DNA polymerase from Thermus thermophilus and DNA template by photoreactive analogs of dCTP. // Biochemistry (Moscow), Vol. 63, No. 8, 1090-1096 (1998). (In Russian)
Degtyarev S. Kh., Prikhod'ko E.A., Rechkunova N.I., Prikhod'ko G.G., Krasnykh V.N. Biochemical characterization of Vsp I methyltransferase. // Gene, 157, 65-66 (1995).
Degtyarev S. Kh., Prikhod'ko E.A., Rechkunova N.I., Gorbunov. Yu. A. Interaction of Vsp I and Tru9 I restriction endonuclease with synthetic oligonucleotides. // Biochimica et Biophysica Acta, 1172, 89-94 (1993).
Degtyarev S. Kh., Prikhod'ko E.A., Prikhod'ko G.G., Krasnykh V.N. Vsp I methylase belongs to m6A-gamma class of adenine methylases. // Nucleic Acids Research, Vol. 21, No 8., 2015 (1993).
Lebedev L.R., Pustoshilova N.M., Repin V.E., Degtyarev S.Kh. Isolation of restriction endonuclease RsaI from Rhodopseudomonas sphaeroides and study of its properties. // Applied biochemistry and microbiology(Moscow), Vol.27, No.3, 330-337 (1991). (In Russian)
Degtyarev S.Kh., Zernov Yu.P. Type II restriction endonucleases: scheme of possible evolution relations between enzymes with palindrome tetra nucleotide recognition sequences. // Molecular Biology(Moscow), Vol.24, No. 5, 1393-1398 (1990). (In Russian)
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